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KMID : 0367419690120010025
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Journal of Korean Pediatric Society
1969 Volume.12 No. 1 p.25 ~ p.27
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The Effect of Environmental Temperature and Time on C.S.F. Cell Counts
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李學洙/Lee, H.S.
李承郁/劉鎭雨/金潗/Lee, S.W./Yu, J.W./Kim, J.
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Abstract
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The effect of environmental temperature and time on C.S.F. cell counts was studied in specimens from twenty patients with Encephalitis or Meningitis. The following results were obtained;
Percentage of reduction in C.S.F. cell counts
Room temp. (18℃) Refrigerator temp.(4℃)
1 hr 18.37% 1.88%
3 hr 30.83% 2.73%
6 hr 38.07% 6.69%
12hr 50.58% 11.32%
24hr 64.80% 20.49%
Conclusions:
1) There was significantly less reduction in cell count of C.S.F. stored at 4℃(refrigerator temp.) than at 18℃(room temp.) at all time during 24 hours.
2) If C.S.F. specimens are maintained at 18℃(room temp.), it is recommanded that cell count be done within 60 minutes or less after lumbar puncture to assure accuracy.
3) If it is impossible to study the C.S.F. cell count within minutes after lumbar puncture, it is recommanded that the C.S.F. be stored immediately at 4℃(refrigerator temp.) and cell count done before twelve hours.
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